An antimicrobial peptide-immobilized nanofiber mat with superior performances than the commercial silver-containing dressing

Abstract

Silver-containing dressings are widely used for the treatment of infected wounds in clinics, but the potential risks of heavy metals are still a common concern. In this study, we prepared a type of electrospun starch nanofiber mat containing the antimicrobial peptide ε-poly-lysine (Starch-EPL) and compared its relevant properties with a representative silver-containing dressing 3M™ Tegaderm™ Alginate Ag (Alginate-Ag). SEM, FTIR and EDAX results show the two samples have similar fiber structures and are loaded with antibacterial agents. The comparison results indicate that the Starch-EPL nanofiber mat has equivalent permeability and absorbency with Alginate-Ag but higher mechanical property and wettability. Moreover, the Starch-EPL nanofiber mat has comparable antibacterial activity against both Gram-negative and Gram-positive bacteria with Alginate-Ag, but markedly better biocompatibility than that. The Starch-EPL nanofiber mat can inhibit the growth of bacteria for at least 14 days by sustainably releasing EPL, showing great potential as a long-term antibacterial dressing. All these results demonstrate that the Starch-EPL nanofiber mat may be a good candidate to replace the traditional silver-containing dressings.

Keywords: Antibacterial activity; Antimicrobial peptide; Biocompatibility; Electrospun nanofiber; Wound dressing.