Using MgO nanoparticles as a potential platform to precisely load and steadily release Ag ions for enhanced osteogenesis and bacterial killing

Si Yang; Luxin Liang; Ling Liu; Yong Yin; Yong Liu; Guanghua Lei; Kun Zhou; Qianli Huang; Hong Wu

doi:10.1016/j.msec.2020.111399

Using MgO nanoparticles as a potential platform to precisely load and steadily release Ag ions for enhanced osteogenesis and bacterial killing

Mater Sci Eng C Mater Biol Appl. 2021 Feb:119:111399. doi: 10.1016/j.msec.2020.111399. Epub 2020 Aug 22.

Authors

Si Yang¹, Luxin Liang¹, Ling Liu², Yong Yin³, Yong Liu¹, Guanghua Lei⁴, Kun Zhou⁵, Qianli Huang⁶, Hong Wu⁷

Affiliations

¹ State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China.
² Hepatobiliary and Pancreatic Surgery Department, Xiangya Hospital, Central South University, Changsha 410008, PR China.
³ State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China; School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore.
⁴ Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008, PR China.
⁵ School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore.
⁶ State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China. Electronic address: hql1990@163.com.
⁷ State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China. Electronic address: hwucsu@csu.edu.cn.

PMID: 33321576
DOI: 10.1016/j.msec.2020.111399

Abstract

Bio-functional fillers including bio-ceramic, degradable metallic and composite particles are commonly introduced into bone tissue engineering (BTE) scaffolds to endow the materials with specific biological functions for enhanced bone defect therapy. In this work, MgO nanoparticles (NPs) were employed as a potential platform for precise loading and sustained release of Ag⁺. The results showed that MgO NPs possessed strong adsorption capacity (almost 100%) towards Ag⁺ in AgNO₃ solutions with different concentrations (0.1, 1 and 10 mM). After the adsorption of Ag⁺ in AgNO₃ solutions, cube-shaped MgO NPs transformed to lamella-structured nano-composites (NCs) composed of Mg(OH)₂ and Ag₂O, which were referred as MgO-xAg (x = 0.1, 1 or 10) NCs depending on the employed concentration of AgNO₃ solution. After being suspended in distilled water, as-prepared positively charged NCs underwent a fast degradation process during the initial 4 days. From day 4 and 14, steady release behaviors of Mg²⁺ and/or Ag⁺ from the NCs were noticed. With the lowest loading amount of Ag⁺, MgO-0.1Ag NCs did not exhibit significant modulatory effect on SaOS-2 cell response. On the contrary, MgO-10Ag NCs loaded with the highest amount of Ag⁺ showed significant cyto-toxicity towards SaOS-2 cells. With appropriate amount of Ag⁺ loading, MgO-1Ag NCs showed significantly stimulatory effects on SaOS-2 cell proliferation and differentiation. This is evidenced by the enhanced cell viability, alkaline phosphatase (ALP) activity and collagen (COL) production as well as the gene expressions of ALP, COL and osteoprotegerin (OPG) in MgO-1Ag group. Moreover, MgO-1Ag exhibited strong bactericidal capacity against both Escherichia coli and Staphylococcus aureus. Together, the results indicate that MgO could be employed as a potential platform for precise loading and sustained release of Ag⁺. MgO-1Ag NCs are promising to be used as bio-functional fillers in BTE scaffolds for simultaneously promoted osteogenesis and bacterial killing.

Keywords: Bacteria; Bone repair; Magnesium oxide; Nanocomposites; Osteoblast; Silver.

MeSH terms

Anti-Bacterial Agents / pharmacology
Ions / pharmacology
Magnesium Oxide / pharmacology
Metal Nanoparticles*
Nanoparticles*
Osteogenesis
Silver / pharmacology

Substances

Anti-Bacterial Agents
Ions
Magnesium Oxide
Silver