Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA

Alejandro Monserrat García-Alegría; Iván Anduro-Corona; Cinthia Jhovanna Pérez-Martínez; María Alba Guadalupe Corella-Madueño; María Lucila Rascón-Durán; Humberto Astiazaran-Garcia

doi:10.1155/2020/8896738

Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA

Int J Anal Chem. 2020 Nov 29:2020:8896738. doi: 10.1155/2020/8896738. eCollection 2020.

Authors

Alejandro Monserrat García-Alegría¹, Iván Anduro-Corona², Cinthia Jhovanna Pérez-Martínez¹, María Alba Guadalupe Corella-Madueño¹, María Lucila Rascón-Durán¹, Humberto Astiazaran-Garcia^{1

2}

Affiliations

¹ Universidad de Sonora, Departamento de Ciencias Químico Biológicas, Hermosillo, Sonora CP 83000, Mexico.
² Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD AC), Coordinación de Nutrición, Hermosillo, Sonora CP 83304, Mexico.

Abstract

This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2-4°C).