Long noncoding RNA expression profiles in intermittent parathyroid hormone induced cementogenesis

Genomics. 2021 Jan;113(1 Pt 1):217-228. doi: 10.1016/j.ygeno.2020.12.012. Epub 2020 Dec 10.

Abstract

The aim of this study was to explore the involvement of long noncoding RNAs (lncRNAs) during intermittent parathyroid hormone (PTH) induced cementogenesis. Expression profiles of lncRNAs and mRNAs were obtained using high-throughput microarray. Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and coding-noncoding gene coexpression networks construction were performed. We identified 190 lncRNAs and 135 mRNAs that were differentially expressed during intermittent PTH-induced cementogenesis. In this process, the Wnt signaling pathway was negatively regulated, and eight lncRNAs were identified as possible core regulators of Wnt signaling. Based on the results of microarrray analysis, we further verified the repressed expression of Wnt signaling crucial components β-catenin, APC and Axin2. Above all, we speculated that lncRNAs may play important roles in PTH-induced cementogenesis via the negative regulation of Wnt pathway.

Keywords: Cementoblast; Long noncoding RNA; Microarray; Parathyroid hormone; Wnt signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenomatous Polyposis Coli Protein
  • Animals
  • Axin Protein / genetics
  • Axin Protein / metabolism
  • Cell Line
  • Cementogenesis*
  • Dental Cementum / metabolism
  • Mice
  • Osteoblasts / metabolism
  • Parathyroid Hormone / metabolism*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Transcriptome
  • Wnt Signaling Pathway*
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • Adenomatous Polyposis Coli Protein
  • Axin Protein
  • Axin2 protein, mouse
  • Parathyroid Hormone
  • RNA, Long Noncoding
  • adenomatous polyposis coli protein, mouse
  • beta Catenin