Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir

Ecol Evol. 2020 Oct 27;10(23):13403-13411. doi: 10.1002/ece3.6944. eCollection 2020 Dec.

Abstract

To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next-generation sequencing. Three hundred and sixty-five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.

Keywords: African tree species; Pterocarpus erinaceus; genetic diversity; next‐generation sequencing (NGS); nuclear microsatellites.