Identification of chalcone synthase genes and their expression patterns reveal pollen abortion in cotton

Xiangjun Kong; Aziz Khan; Zhiling Li; Jingyi You; Fazal Munsif; Haodong Kang; Ruiyang Zhou

doi:10.1016/j.sjbs.2020.08.013

Identification of chalcone synthase genes and their expression patterns reveal pollen abortion in cotton

Saudi J Biol Sci. 2020 Dec;27(12):3691-3699. doi: 10.1016/j.sjbs.2020.08.013. Epub 2020 Aug 19.

Authors

Xiangjun Kong^{1

2}, Aziz Khan², Zhiling Li², Jingyi You², Fazal Munsif², Haodong Kang², Ruiyang Zhou^{1

2}

Affiliations

¹ College of Life Sciences and Technology, Guangxi University, Nanning, 530005, PR China.
² Key Laboratory of Plant Genetic and Breeding, College of Agriculture, Guangxi University, Nanning 530005, PR China.

Abstract

Chalcone synthase (CHS) is a key enzyme and producing flavonoid derivatives as well play a vital roles in sustaining plant growth and development. However, the systematic and comprehensive analysis of CHS genes in island cotton (G. barbadense) has not been reported yet especially response to cytoplasmic male sterility (CMS). To fill this knowledge gap, a genome-wide investigation of CHS genes were studied in island cotton. A total of 20 GbCHS genes were identified and grouped into five GbCHSs. The gene structure analysis revealed that most of GbCHS genes consisted of two exons and one intron, and 20 motifs were identified. Twenty five pairs duplicated events (12 GbCHS genes) were identified including 23 segmental duplication pairs and two tandem duplication events, representing that GbCHS gene family amplification mainly owned to segmental duplication events and evolving slowly. Gene expression analysis exhibited that the GbCHS family genes presented a diversity expression patterns in various organs of cotton. Coupled with functional predictions and gene expression, the abnormal expression of GbCHS06, 10, 16 and 19 might be associated with pollen abortion of CMS line in island cotton. Conclusively, GbCHS genes exhibited diversity and conservation in many aspects, which will help to better understand functional studies and a reference for CHS research in island cotton and other plants.

Keywords: CHS, Chalcone synthase; CMS, cytoplasmic male sterility; Chalcone synthase; Cytoplasmic male sterility; Gene family; Island cotton; qRT-PCR, quantitative reverse transcribed PCR.