FRET-based fluorometry assay for curcumin detecting using PVP-templated Cu NCs

Zhixia Yao; Hanmeng Liu; Yaosheng Liu; Yongxing Diao; Guangxing Hu; Qifang Zhang; Zhuang Li

doi:10.1016/j.talanta.2020.121741

FRET-based fluorometry assay for curcumin detecting using PVP-templated Cu NCs

Talanta. 2021 Feb 1;223(Pt 2):121741. doi: 10.1016/j.talanta.2020.121741. Epub 2020 Oct 8.

Authors

Zhixia Yao¹, Hanmeng Liu¹, Yaosheng Liu¹, Yongxing Diao¹, Guangxing Hu¹, Qifang Zhang², Zhuang Li³

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, 230026, China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, 230026, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zli@ciac.ac.cn.

PMID: 33298267
DOI: 10.1016/j.talanta.2020.121741

Abstract

A novel fluorometric strategy is proposed for detecting curcumin by polyvinyl pyrrolidone-templated Cu NCs (PVP-Cu NCs) as a fluorescent probe which exhibits excitation/emission peaks at 380/510 nm. The fluorescent excitation and emission spectra of PVP-Cu NCs have a striking overlap with the UV-vis spectrum of curcumin, and the fluorescence lifetime of PVP-Cu NCs decreases after the addition of curcumin. Curcumin leads to fluorescence quenching based on fluorescence resonance energy transfer. This method allows for the determination of curcumin in the range of 0.1-10 μg mL^-1 and the detection limit is 21 ng mL^-1. Furthermore, this method displays good selectivity and is successfully applied for real sample analysis.

Keywords: Copper nanoclusters; Curcumin determination; Fluorescence resonance energy transfer; Fluorometry; PVP.