Uptake of cephalexin by lettuce, celery, and radish from water

Abstract

The introduction of pharmaceuticals into agricultural lands from the application of biosolids and animal manure, and irrigation with treated wastewater has led to concern for animal and human health after the ingestion of pharmaceutical-tainted agricultural products. In this study, the uptake and accumulation of cephalexin, a commonly prescribed antibiotic, was compared in three common vegetables (lettuce, celery, and radish) grown in nutrient solution for 144 h. During the uptake experiments, cephalexin concentration in the nutrient solution decreased in the order of radish > celery > lettuce, while the accumulation of cephalexin in vegetable roots followed the rank of lettuce > celery > radish. The accumulation of cephalexin was below the limit of detection in radish roots. No accumulation of cephalexin was observed in the shoots of all three vegetables. The behaviors of cephalexin in vivo were further elucidated using in vitro measurements of cephalexin sorption by vegetable roots and transformation in plant enzyme extracts. The affinity of cephalexin to lettuce > celery > radish roots, and the respective sorption coefficients of 687, 303, and 161 mL g^-1, coupled to the transformation of cephalexin in root enzyme extracts with estimated reaction rate constants of 0.020, 0.027 and 0.024 hr^-1 for lettuce, celery and radish, could help elucidate the accumulation observed in the in vivo experiments. Overall, sorption by plant roots (affinity) and reaction with plant enzymes could collectively influence the uptake and accumulation of cephalexin in vegetables.

Keywords: Accumulation; Enzyme reaction; Pharmaceuticals; Plant uptake; Sorption; Vegetables.