Red/NIR neutral BODIPY-based fluorescent probes for lighting up mitochondria

Jian Yang; Ran Zhang; Yue Zhao; Jiangwei Tian; Shifa Wang; Claude P Gros; Haijun Xu

doi:10.1016/j.saa.2020.119199

Red/NIR neutral BODIPY-based fluorescent probes for lighting up mitochondria

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 5:248:119199. doi: 10.1016/j.saa.2020.119199. Epub 2020 Nov 13.

Authors

Jian Yang¹, Ran Zhang², Yue Zhao¹, Jiangwei Tian³, Shifa Wang¹, Claude P Gros⁴, Haijun Xu⁵

Affiliations

¹ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
² State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
³ State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: jwtian@cpu.edu.cn.
⁴ ICMUB (UMR CNRS 6302), Université Bourgogne Franche-Comté, 21000 Dijon, France. Electronic address: Claude.Gros@u-bourgogne.fr.
⁵ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address: xuhaijun@njfu.edu.cn.

PMID: 33293228
DOI: 10.1016/j.saa.2020.119199

Abstract

Two mitochondrial-targeted fluorescent probes, 2 and 3, based on a BODIPY scaffold and one or two piperidinyl groups were easily synthesized by Knoevenagel condensation. The biological imaging applications of the two probes 2 and 3 in live HepG2 cells reveal that these two probes display excellent mitochondrial imaging ability. Thus, these probes appear as promising tools for visualization of mitochondrial within live cells.

Keywords: BODIPY; Fluorescent probes; Imaging; Mitochondria.

MeSH terms

Boron Compounds*
Diagnostic Imaging
Fluorescent Dyes*
Mitochondria

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes