Nuclearly-encoded CBP1 interacts with the 5' end of mitochondrial cytochrome b pre-mRNA

Curr Genet. 1987;12(6):391-7. doi: 10.1007/BF00434815.

Abstract

CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Nucleus / metabolism*
  • Cytochrome b Group / genetics*
  • Fungal Proteins / genetics*
  • Genes*
  • Genes, Fungal*
  • Genotype
  • Mitochondria / metabolism*
  • RNA Precursors / genetics*
  • RNA, Messenger / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Cytochrome b Group
  • Fungal Proteins
  • RNA Precursors
  • RNA, Messenger