Abstract
Asthma is considered as a general term for various chronic inflammatory diseases of the respiratory tract. Growing evidences have supported that microRNAs were involved in mediating cell proliferation, migration, and other cellular functions. MiR-149 has been found to take part in the development of various cancers. However, whether miR-149 participated in the proliferation and migration of transforming growth factor beta 1 (TGF-β1)-induced airway smooth muscle cells was still unknown. In this study, the expression level of miR-149 in human airway smooth muscle cells (ASMCs) was decreased after TGF-β1 treatment in vitro. Additionally, the over-expression of miR-149 obviously suppressed proliferation and migration in human ASMCs. Besides, we found that overexpression of miR-149 could inhibit the expression of transient receptor potential melastatin 7 (TRPM7) both in protein and gene levels. Furthermore, we demonstrated that miR-149 could inhibit the cell proliferation and migration in human ASMCs by targeting TRPM7 through modulating mitogen-activated protein kinases (MAPKs) signaling pathway. Taken together, we strongly supported that miR-149 might be a key inhibitor of asthma by targeting TRMP7. Therefore, our finding suggests a promising biomarker for the development of further targeted therapies for asthma.
MeSH terms
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Antagomirs / genetics
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Antagomirs / metabolism
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Base Pairing
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Base Sequence
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Cell Line
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Cell Movement / drug effects
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Cell Proliferation / drug effects
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Gene Expression Regulation
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HEK293 Cells
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Humans
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MAP Kinase Kinase 4 / genetics
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MAP Kinase Kinase 4 / metabolism
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MicroRNAs / agonists
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Mitogen-Activated Protein Kinase 1 / genetics
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / genetics
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Mitogen-Activated Protein Kinase 3 / metabolism
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Myocytes, Smooth Muscle / cytology
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Myocytes, Smooth Muscle / drug effects*
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Myocytes, Smooth Muscle / metabolism
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Oligoribonucleotides / genetics
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Oligoribonucleotides / metabolism
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Protein Serine-Threonine Kinases / genetics*
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Protein Serine-Threonine Kinases / metabolism
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Proto-Oncogene Proteins c-akt / genetics
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Proto-Oncogene Proteins c-akt / metabolism
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Respiratory System / cytology
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Respiratory System / drug effects
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Respiratory System / metabolism
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Signal Transduction / genetics*
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TRPM Cation Channels / genetics*
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TRPM Cation Channels / metabolism
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Transforming Growth Factor beta1 / genetics
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Transforming Growth Factor beta1 / metabolism
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Transforming Growth Factor beta1 / pharmacology*
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p38 Mitogen-Activated Protein Kinases / genetics
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Antagomirs
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MIRN149 microRNA, human
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MicroRNAs
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Oligoribonucleotides
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TGFB1 protein, human
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TRPM Cation Channels
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Transforming Growth Factor beta1
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-akt
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TRPM7 protein, human
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MAPK1 protein, human
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MAPK3 protein, human
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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p38 Mitogen-Activated Protein Kinases
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MAP Kinase Kinase 4