Abstract
After germination, plants determine their morphogenesis, such as hypocotyl elongation and cotyledon opening, by responding to various wavelengths of light (photomorphogenesis). Cryptochrome is a blue-light photoreceptor that controls de-etiolation, stomatal opening and closing, flowering time, and shade avoidance. Successful incorporation of these phenotypes as indicators into a chemical screening system results in faster selection of candidate compounds. Here, we describe phenotypic screening for the blue-light response of Arabidopsis thaliana seedling and the resulting process that clarifies that the compound obtained in the screening is an inhibitor of cryptochromes.
Keywords:
Cryptochrome; Image processing; Phenotypic screen; Photoreceptor; photomorphogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis / radiation effects
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Cell-Free System
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Cotyledon / anatomy & histology
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Cotyledon / drug effects
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Cotyledon / radiation effects
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Cryptochromes / antagonists & inhibitors*
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Cryptochromes / metabolism
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Cryptochromes / radiation effects
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Culture Media
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Hypocotyl / anatomy & histology
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Hypocotyl / drug effects
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Hypocotyl / radiation effects
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Image Processing, Computer-Assisted
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Light
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Phenotype
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Protein Biosynthesis / drug effects
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Protein Biosynthesis / radiation effects
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Recombinant Proteins / biosynthesis
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Seedlings / drug effects
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Seedlings / radiation effects
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Small Molecule Libraries / analysis*
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Small Molecule Libraries / pharmacology
Substances
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Cryptochromes
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Culture Media
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Recombinant Proteins
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Small Molecule Libraries