An innovative prussian blue nanocubes decomposition-assisted signal amplification strategy suitable for competitive lateral flow immunoassay to sensitively detect aflatoxin B1

Abstract

A prussian blue nanocubes decomposition-assisted signal amplification strategy for competitive lateral flow immunoassay (PBNCD-SALFIA) was innovatively proposed to analyze aflatoxin B₁ (AFB₁) in foodstuffs. The signal was amplified on account of the PBNCs can degrade and fade under alkaline condition, which could weak the color of the test- and control-lines, thereby improving the sensitivity of the sensor. The strategy was technically-simple, just using NaOH as an amplifier can realize signal amplification in the competition assay. Under optimized conditions, the enhanced strip exhibited excellent specificity and sensitivity in AFB₁ monitoring with a detection limit of 23 pg/mL, which was approximately 4- and 8-folds lower than those of PBNCs-LFIA (90 pg/mL) and conventional gold nanoparticles-LFIA (175 pg/mL), respectively. Taking the advantage of the color-fading, this platform revealing the lowest detectable concentration of 0.184, 0.368 and 0.184 μg/kg for AFB₁ in corn, peanut and pumpkin seed within 58 min, separately, showing reliable results.

Keywords: Aflatoxin B1; Detection; Lateral flow immunoassay; Prussian blue nanocubes; Signal amplification.