Antioxidant responses of the mussel Mytilus coruscus co-exposed to ocean acidification, hypoxia and warming

Fahim Ullah Khan; Hui Chen; Huaxin Gu; Ting Wang; Sam Dupont; Hui Kong; Yueyong Shang; Xinghuo Wang; Weiqun Lu; Menghong Hu; Youji Wang

doi:10.1016/j.marpolbul.2020.111869

Antioxidant responses of the mussel Mytilus coruscus co-exposed to ocean acidification, hypoxia and warming

Mar Pollut Bull. 2021 Jan:162:111869. doi: 10.1016/j.marpolbul.2020.111869. Epub 2020 Nov 27.

Authors

Fahim Ullah Khan¹, Hui Chen², Huaxin Gu³, Ting Wang³, Sam Dupont⁴, Hui Kong³, Yueyong Shang³, Xinghuo Wang³, Weiqun Lu³, Menghong Hu⁵, Youji Wang⁶

Affiliations

¹ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Fisheries and Aquaculture Programme, Department of Zoology, Quaid-i-Azam University, Islamabad, Pakistan.
² Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China.
³ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
⁴ Department of Biological and Environmental Sciences, Sven Lovén Centre for Marine Infrastructure - Kristineberg, University of Gothenburg, Fiskebäckskil, Sweden.
⁵ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address: mhhu@shou.edu.cn.
⁶ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address: youjiwang2@gmail.com.

PMID: 33256964
DOI: 10.1016/j.marpolbul.2020.111869

Abstract

In the present study, the combined effects of pH, dissolved oxygen (DO) and temperature levels on the antioxidant responses of the mussel Mytilus coruscus were evaluated. Mussels were exposed to two pH (8.1, 7.7-acidification), two DO (6 mg L^-1, 2 mg L^-1-hypoxia) and two temperature levels (20 °C, 30 °C-warming) for 30 days. SOD, CAT, MDA, GPx, GSH, GST, TAOC, AKP, ACP, GPT, AST levels were measured in the gills of mussels. All tested biochemical parameters were altered by these three environmental stressors. Values for all the test parameters except GSH first increased and then decreased at various experimental treatments during days 15 and 30 as a result of acidification, hypoxia and warming. GSH content always increased with decreased pH, decreased DO and increased temperature. PCA showed a positive correlation among all the measured biochemical indexes. IBR results showed that M. coruscus were adversely affected by reduced pH, low DO and elevated temperature.

Keywords: Antioxidant enzyme; Hypoxia; Ocean acidification; Oxidative stress; Thermal stress.

MeSH terms

Animals
Antioxidants
Hydrogen-Ion Concentration
Hypoxia
Mytilus*
Oceans and Seas
Seawater

Substances

Antioxidants