A bifunctionalized NiCo2O4-Au composite: Intrinsic peroxidase and oxidase catalytic activities for killing bacteria and disinfecting wound

Abstract

A NiCo₂O₄-Au composite was fabricated following the calcination-reduction method and testified to exhibit the intrinsic high peroxidase- and oxidase-like activities. The composite could activate a low level of H₂O₂ (100 μM) to kill E. coli and S. aureus. NiCo₂O₄-Au composite could be easily separated by an external magnet from the media and reused several times. NiCo₂O₄-Au composite could also effectively damage the existing biofilms and prevent the formation of new biofilms. The electron spin resonance tests showed that NiCo₂O₄-Au composite catalyzed H₂O₂ into reactive oxygen species (ROS), mainly including OH, O₂^-, and ¹O₂; while the oxidase-like activity of NiCo₂O₄-Au also stemmed from the ROS formation in the absence of H₂O₂. The radical trapping experiment confirmed that OH and ¹O₂ were the main radicals in the antibacterial process for NiCo₂O₄-Au in the presence of H₂O₂. A NiCo₂O₄-Au based Band-Aid was also designed, which exhibited high anti-infective and wound-healing properties. This study has demonstrated that NiCo₂O₄-Au composite can be a promising antibacterial agent in environmental and clinical applications.

Keywords: Antibacterial; Catalysis; Nanozyme; NiCo(2)O(4)-Au; ROS.