Synchrotron Radiation-Based FTIR Microspectroscopic Imaging of Traumatically Injured Mouse Brain Tissue Slices

Yuansen Guo; Tunan Chen; Shi Wang; Xiaojie Zhou; Hua Zhang; Dandan Li; Ning Mu; Mingjie Tang; Meidie Hu; Dongyun Tang; Zhongbo Yang; Jiajia Zhong; Yuzhao Tang; Hua Feng; Xuehua Zhang; Huabin Wang

doi:10.1021/acsomega.0c03285

Synchrotron Radiation-Based FTIR Microspectroscopic Imaging of Traumatically Injured Mouse Brain Tissue Slices

ACS Omega. 2020 Oct 16;5(46):29698-29705. doi: 10.1021/acsomega.0c03285. eCollection 2020 Nov 24.

Authors

Yuansen Guo^{1

2}, Tunan Chen³, Shi Wang³, Xiaojie Zhou⁴, Hua Zhang^{1

5}, Dandan Li¹, Ning Mu³, Mingjie Tang^{1

2}, Meidie Hu¹, Dongyun Tang^{1

5}, Zhongbo Yang¹, Jiajia Zhong⁴, Yuzhao Tang⁴, Hua Feng³, Xuehua Zhang⁶, Huabin Wang¹

Affiliations

¹ Center of Applied Physics & Chongqing Engineering Research Center of High-Resolution and Three-Dimensional Dynamic Imaging Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
² University of Chinese Academy of Sciences, Beijing 100049, China.
³ Department of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
⁴ National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
⁵ Chongqing School, University of Chinese Academy of Sciences, Chongqing 400714, China.
⁶ Department of Chemical & Materials Engineering, University of Alberta, Alberta T6G1H9, Canada.

Abstract

Traumatic brain injury (TBI) is a health problem of global concern because of its serious adverse effects on public health and social economy. A technique that can be used to precisely detect TBI is highly demanded. Here, we report on a synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopic imaging technique that can be exploited to identify TBI-induced injury by examining model mouse brain tissue slices. The samples were first examined by conventional histopathological techniques including hematoxylin and eosin (H&E) staining and 2,3,5-triphenyltetrazolium chloride staining and then spectroscopically imaged by SR-FTIR. SR-FTIR results show that the contents of protein and nucleic acid in the injured region are lower than their counterparts in the normal region. The injured and normal regions can be unambiguously distinguished from each other by the principle component analysis of the SR-FTIR spectral data corresponding to protein or nucleic acid. The images built from the spectral data of protein or nucleic acid clearly present the injured region of the brain tissue, which is in good agreement with the H&E staining image and optical image of the sample. Given the label-free and fingerprint features, the demonstrated method suggests potential application of SR-FTIR spectroscopic mapping for the digital and intelligent diagnosis of TBI by providing spatial and chemical information of the sample simultaneously.