Immobilization altering the growth behavior, ammonium uptake and amino acid synthesis of Chlorella vulgaris at different concentrations of carbon and nitrogen

Abstract

Nitrogen recycling by microalgae has aroused considerable attention. In this study, immobilized Chlorellavulgaris with 5-day mixotrophic cultivation to recover ammonium (NH₄⁺-N) were systematically investigated under various sodium acetate (CH₃COONa) and ammonium chloride (NH₄Cl) concentrations, and evaluated by comparison with suspended cells. The results revealed that, unlike suspended cells, NH₄⁺-N uptake by immobilized cells was not in direct proportion to chemical oxygen demand (COD) concentrations. The immobilized cells to NH₄⁺-N uptake was all inferior to that of suspended cells, presenting the maximum rate of 68.92% in group of 30 mg/L NH₄⁺-N and 200 mg/L COD. Free amino acids in immobilized cells such as glutamate (Glu), arginine (Arg), proline (Pro) and leucine (Leu) were more sensitive to NH₄⁺-N assimilation, as higher values observed by suspended cells. Low carbon-nitrogen (C/N) ratio showed remarkable benefits to amino acid synthesis. These results could provide a reference for manipulating the algal system and biomass accumulation.

Keywords: Amino acid composition; Ammonium; Chlorella vulgaris; Immobilization; Mixotrophic culture.