Abstract
Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid - IAA; 2,4-Dichlorophenoxyacetic acid - 2,4-D; kinetin - KIN; 6-Benzylaminopurine - BAP) combinations to compare different approaches to callus induction directly from the seeds of Nicotiana tabacum. Callus formation was observed up to 4 weeks after sowing and the most effective were 0.5 mg/L of 2,4-D with 0.25 mg/L of BAP and 2 mg/L 2,4-D with 1 mg/L of BAP. The calli were green, photosynthetically active and after 6 weeks of growth, no stress symptoms (estimated on the basis of fluorescence of chlorophyll a in photosystem II) were noticed.
MeSH terms
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2,4-Dichlorophenoxyacetic Acid / pharmacology*
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Benzyl Compounds / pharmacology*
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Chlorophyll A / biosynthesis
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Germination / drug effects
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Germination / physiology
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Indoleacetic Acids / pharmacology*
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Kinetin / pharmacology*
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Mesophyll Cells / cytology
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Mesophyll Cells / drug effects
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Mesophyll Cells / metabolism
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Nicotiana / cytology
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Nicotiana / drug effects*
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Nicotiana / metabolism
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Photosynthesis / drug effects
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Photosynthesis / physiology
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Plant Growth Regulators / pharmacology*
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Purines / pharmacology*
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Seedlings / cytology
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Seedlings / drug effects
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Seedlings / metabolism
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Seeds / cytology
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Seeds / drug effects
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Seeds / metabolism
Substances
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Benzyl Compounds
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Indoleacetic Acids
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Plant Growth Regulators
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Purines
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2,4-Dichlorophenoxyacetic Acid
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indoleacetic acid
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benzylaminopurine
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Kinetin
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Chlorophyll A