Background: An interlaboratory study was conducted to test a published, peer-reviewed manuscript in the Journal of AOAC INTERNATIONAL Vol 98, No. 3, 2015, "Quantitation of Chloramphenicol and Nitrofuran Metabolites in Aquaculture Products Using Microwave-Assisted Derivatization, Automated Solid-Phase Extraction, and LC-MS/MS."
Objective: The purpose of this study was to demonstrate the performance of the method in shrimp, cobia, and croaker matrices.
Method: Three U.S. Food and Drug Administration laboratories participated in the collaborative study. The laboratories tested matrix blanks and laboratory-fortified matrix blanks at various levels in three separate matrices. The method evaluation included determination of the LOQ, accuracy, and precision.
Results: The reproducibility and repeatability of the RSD, % levels for matrix spikes fortified below the action level were < 10% for all residues in shrimp, < 13% for all residues in cobia except for 3-amino-2-oxazolidinone which was ≤ 22%, and < 16% for croaker. The RSD, % levels for all other spikes in the study were < 14%. Average percent recoveries for all matrices ranged from 81.6% - 102%.
Conclusions: The study demonstrated that the method is acceptable for use for the combined determination of chloramphenicol and nitrofuran metabolites in the study matrices.
Highlights: The study showed acceptable quantitation for the high-throughput chloramphenicol and nitrofuran metabolites method.
AOAC INTERNATIONAL 2020. This work is written by a US Government employee and is in the public domain in the US.