Eco-friendly silver nanoparticles (AgNPs) fabricated by green synthesis using the crude extract of marine polychaete, Marphysa moribidii: biosynthesis, characterisation, and antibacterial applications

Nur Syakirah Rabiha Rosman; Noor Aniza Harun; Izwandy Idris; Wan Iryani Wan Ismail

doi:10.1016/j.heliyon.2020.e05462

Eco-friendly silver nanoparticles (AgNPs) fabricated by green synthesis using the crude extract of marine polychaete, Marphysa moribidii: biosynthesis, characterisation, and antibacterial applications

Heliyon. 2020 Nov 12;6(11):e05462. doi: 10.1016/j.heliyon.2020.e05462. eCollection 2020 Nov.

Authors

Nur Syakirah Rabiha Rosman^{1

2}, Noor Aniza Harun^{2

3}, Izwandy Idris⁴, Wan Iryani Wan Ismail^{1

2}

Affiliations

¹ Cell Signalling and Biotechnology Research Group (CeSBTech), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
² Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
³ Advance NanoMaterials (ANOMA) Research Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
⁴ South China Sea Repository and Reference Centre, Institute of Oceanography and Environment (INOS), Universiti Malaysia Terengganu, Terengganu, Malaysia.

Abstract

The non-hazardous silver nanoparticles (AgNPs) synthesised using the extract of a biological organism has gained widespread attention for various applications, mainly in healthcare. This study aimed at synthesising AgNPs using the aqueous extract of Marphysa moribidii (Annelida, Polychaeta) and to evaluate their antibacterial activities. AgNPs were synthesised in response to silver nitrate (AgNO₃) with polychaete crude extract for 24 h incubation; the polychaete crude extract acted as both reducing and stabilising agents. The presence of biosynthesised AgNPs was confirmed by an analysis of colour variations from pinkish to yellowish-brown, as well as the appearance of surface Plasmon resonance (SPR) bands at 398-400 nm using ultraviolet-visible spectroscopy. Biosynthesised AgNPs were characterised by dynamic light scattering, scanning electron microscope, transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. Biosynthesised AgNPs showed a significant effect (p < 0.05) on Gram-positive bacteria (Staphylococcus aureus and S . epidermidis) and Gram-negative bacteria (Escherichia coli, Klebsiella pnemoniae, Salmonella typhimurium, Serratia sp., Shigella sonnei, and Pseudomonas aeruginosa). Thus, the crude extract of M. moribidii has a potential as a reducing agent for the development of future nanometal-based antibacterial agent, AgNPs, for the treatment of infectious diseases caused by pathogenic bacteria.

Keywords: Antibacterial study; Biotechnology; Green synthesis; Marphysa moribidii; Materials science; Nanobiotechnology; Nanotechnology; Polychaetes; Silver nanoparticles.