RPA, RFWD3 and BRCA2 at stalled forks: a balancing act

Haohui Duan; Shailja Pathania

doi:10.1080/23723556.2020.1801089

RPA, RFWD3 and BRCA2 at stalled forks: a balancing act

Mol Cell Oncol. 2020 Aug 23;7(6):1801089. doi: 10.1080/23723556.2020.1801089. eCollection 2020.

Authors

Haohui Duan^{1

2}, Shailja Pathania^{1

2}

Affiliations

¹ Center for Personalized Cancer Therapy, University of Massachusetts, Boston, MA, USA.
² Department of Biology, University of Massachusetts, Boston, MA, USA.

Abstract

We recently identified E3 ligase RFWD3 as a modulator of stalled fork stability in BRCA2-deficient cells. We also show that BRCA1 might function upstream of BRCA2 during fork repair and that blocking fork degradation by depleting MRE11 does not guarantee fork repair. These findings provide new insights into the workings of BRCA1 and BRCA2 in the stalled fork repair pathway.

Keywords: BRCA1; BRCA2; MRE11; RFWD3; RPA ubiquitination; SMARCAL1; replication stress.

Abstract

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