Synthesis and cytotoxic analysis of thiolated xylose derivatives decorated on gold nanoparticles

Angus Shiue; Jenn-Han Chen; Chia-Ying Chang; Shu-Mei Chang; Kuo-Yuan Hwa; Kai-Yen Chin; Graham Leggett

doi:10.1016/j.btre.2020.e00549

Synthesis and cytotoxic analysis of thiolated xylose derivatives decorated on gold nanoparticles

Biotechnol Rep (Amst). 2020 Nov 1:28:e00549. doi: 10.1016/j.btre.2020.e00549. eCollection 2020 Dec.

Authors

Angus Shiue¹, Jenn-Han Chen², Chia-Ying Chang¹, Shu-Mei Chang^{1

3

4}, Kuo-Yuan Hwa^{1

3

5}, Kai-Yen Chin¹, Graham Leggett⁶

Affiliations

¹ Graduate Institute of Organic and Polymeric Materials, National Taipei University of Technology, Taipei, Taiwan.
² Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan.
³ Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.
⁴ Research and Development Center for Smart Textile Technology, National Taipei University of Technology, Taipei, Taiwan.
⁵ Center for Biomedical Industry, National Taipei University of Technology, Taipei, Taiwan.
⁶ LI-COR Biosciences, Cambridge, England.

Abstract

The rapid development of metal nanoparticles capped by an organic monolayer offers the possibility to create a whole new variety of products with novel characteristic, functions and applications. Among these, nanoparticles covered with carbohydrates (glyconanoparticles) constitute a good bio-mimetic model of carbohydrate presentation at the cell surface and are currently centered on many glycobiological and biomedical applications. In this study, a series of novel D-xylose gold nanoparticles (AuNPs) with linkages of alkyl or polyethylene glycol have been synthesized via D-xylosethiols, forming self-assembled monolayers on gold nanoparticles. The nano-gold solution, two carbohydrate derivatives and modified nano-gold solution were tested for cytotoxicity to check the biocompatibility. The MTT assay on NIH 3T3 cell lines confirmed that all the test materials showed no toxicity with the more than 90 % of cell viability in both low concentration (1 μM) and high concentration (100 μM), compared with the control.

Keywords: Gold nanoparticles; Plasmon resonance; Surface; Xylose.