High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma

Tyler J Alban; Defne Bayik; Alvaro G Alvarado; Harley I Kornblum; Justin D Lathia

doi:10.1007/978-1-0716-1060-2_13

High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma

Methods Mol Biol. 2021:2236:157-175. doi: 10.1007/978-1-0716-1060-2_13.

Authors

Tyler J Alban¹, Defne Bayik¹, Alvaro G Alvarado², Harley I Kornblum², Justin D Lathia³

Affiliations

¹ Department of Cardiovascular and Metabolic Sciences, Cleveland Clinic, Cleveland, OH, USA.
² Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, University of California Los Angeles, Los Angeles, CA, USA.
³ Department of Cardiovascular and Metabolic Sciences, Cleveland Clinic, Cleveland, OH, USA. lathiaj@ccf.org.

PMID: 33237547
DOI: 10.1007/978-1-0716-1060-2_13

Abstract

We will first describe analysis of MDSC subsets from patient tumors with multicolor flow cytometry. The key components of this methodology are to obtain viable single cell suspensions and eliminate red blood cell contamination.

Keywords: CyTOF; Flow cytometry; Glioblastoma (GBM); Immune analysis; Myeloid-derived suppressor cells (MDSCs); Patient tumor; Peripheral blood.

MeSH terms

Brain Neoplasms / pathology*
Cell Membrane / metabolism
Cell Movement*
Cell Separation / methods*
Epitopes / metabolism
Flow Cytometry
Glioblastoma / pathology*
Humans
Myeloid-Derived Suppressor Cells / pathology*
Phenotype
Staining and Labeling
Transcriptome / genetics

Substances

Epitopes