Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays

Methods Mol Biol. 2021:2237:69-82. doi: 10.1007/978-1-0716-1064-0_6.

Abstract

Electrochemistry is a multidisciplinary field encompassing the study of analytes in solution for detection and quantification. For the medical field, this brings opportunities to the clinical practice of disease detection through measurements of disease biomarkers. Specifically, panels of biomarkers offer an important future option that can enable physicians' access to blood, saliva, or urine bioassays for screening diseases, as well as monitoring the progression and response to therapy. Here, we describe the simultaneous detection of eight protein cancer biomarkers in a 30-min assay by a microfluidic electrochemical immunoarray.

Keywords: Amperometry; Biomarkers; ELISA; Immunoarray; Layer-by-layer; Microfluidics; Protein detection.

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods
  • Immunologic Tests / instrumentation
  • Immunologic Tests / methods*
  • Microfluidics / instrumentation
  • Microfluidics / methods*
  • Protein Array Analysis / instrumentation
  • Protein Array Analysis / methods*

Substances

  • Biomarkers, Tumor