The impact of boiling and in vitro human digestion of Solanum nigrum complex (Black nightshade) on phenolic compounds bioactivity and bioaccessibility

S M Moyo; J C Serem; M J Bester; V Mavumengwana; E Kayitesi

doi:10.1016/j.foodres.2020.109720

The impact of boiling and in vitro human digestion of Solanum nigrum complex (Black nightshade) on phenolic compounds bioactivity and bioaccessibility

Food Res Int. 2020 Nov:137:109720. doi: 10.1016/j.foodres.2020.109720. Epub 2020 Sep 22.

Authors

S M Moyo¹, J C Serem², M J Bester², V Mavumengwana³, E Kayitesi⁴

Affiliations

¹ Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Johannesburg, South Africa.
² Department of Anatomy, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Arcadia 0007, Pretoria, South Africa.
³ DST-NRF Centre of Excellence for Biomedical Tuberculosis Research/South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
⁴ Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Johannesburg, South Africa; Department of Consumer and Food Sciences, University of Pretoria, Hatfield, Pretoria, South Africa. Electronic address: eugenie.kayitesi@up.ac.za.

PMID: 33233289
DOI: 10.1016/j.foodres.2020.109720

Abstract

Solanum nigrum complex (Black nightshade) is a wild leafy vegetable with phenolic antioxidant compounds related to the reduction of oxidative stress. Changes in phenolics and bioactivity due to cooking and gastrointestinal digestion of black nightshade were compared to spinach. Predominant compounds of black nightshade were myricetin, quercetin-3-O-robinoside, 3,4-dicaffeoylquinic acid, 3-caffeoylquinic acid, and rutin, which were improved after boiling but reduced after in vitro digestion. Phenolics were reduced after digestion of black nightshade and spinach; however, bioactivity was still retained, especially in preventing oxidative stress in Caco-2 cells. Hence, indicating their potential to reduce oxidative stress related diseases of the digestive tract.

Keywords: Bioaccessibility; Bioactivity; Black nightshade; In vitro digestion; Leafy green vegetables; Phenolic compounds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants
Caco-2 Cells
Digestion
Humans
Phenols
Solanum nigrum*

Substances

Antioxidants
Phenols