Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos

Kwang-Hwan Choi; Dong-Kyung Lee; Jong-Nam Oh; Seung-Hun Kim; Mingyun Lee; Sang-Ho Woo; Dae-Yong Kim; Chang-Kyu Lee

doi:10.1016/j.scr.2020.102093

Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos

Stem Cell Res. 2020 Dec:49:102093. doi: 10.1016/j.scr.2020.102093. Epub 2020 Nov 19.

Authors

Kwang-Hwan Choi¹, Dong-Kyung Lee¹, Jong-Nam Oh¹, Seung-Hun Kim¹, Mingyun Lee¹, Sang-Ho Woo², Dae-Yong Kim², Chang-Kyu Lee³

Affiliations

¹ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea.
² Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
³ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeong Chang 25354, Republic of Korea. Electronic address: leeck@snu.ac.kr.

PMID: 33232901
DOI: 10.1016/j.scr.2020.102093

Abstract

Here, we report in vivo developmental competent pig embryonic stem cells (ESCs) originating from in vitro-fertilized and parthenogenetic embryos cultured in chemically defined culture media. The pig ESC lines expressed pluripotency markers and were capable of forming teratomas following subcutaneous injection into nude mice. These cell lines would be a useful resource for comparative developmental biology and agricultural biotechnology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Cell Line
Embryonic Stem Cells*
Mice
Mice, Nude
Parthenogenesis*
Swine