Generation of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9

Jinbing Li; Yuhang Li; Xiao-Yong Zhan; Xinru Ran; Hung-Fat Tse; Shiying Dang; Yichu Nie; Ke Huang

doi:10.1016/j.scr.2020.102090

Generation of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9

Stem Cell Res. 2020 Dec:49:102090. doi: 10.1016/j.scr.2020.102090. Epub 2020 Nov 17.

Authors

Jinbing Li¹, Yuhang Li², Xiao-Yong Zhan³, Xinru Ran⁴, Hung-Fat Tse⁵, Shiying Dang³, Yichu Nie⁶, Ke Huang⁷

Affiliations

¹ University of Chinese Academy of Sciences, Beijing 100049, China; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
² CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
³ The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China.
⁴ Cardiology Division, Department of Medicine, The University of Hong Kong, Hong Kong SAR, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, China.
⁵ Cardiology Division, Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
⁶ Department of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, Shenzhen 518107, China. Electronic address: nieych3@mail.sysu.edu.cn.
⁷ The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China. Electronic address: huangk65@mail.sysu.edu.cn.

PMID: 33232900
DOI: 10.1016/j.scr.2020.102090

Abstract

GADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been merely documented. To illustrate the function of GADD45A in the human embryonic stem cell biology, we reported a GADD45A knockout human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting. This cell line displayed normal karyotype, pluripotent stem cell marker expression and differentiation potential both in vivo and vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Cycle Proteins
Cell Line*
Gene Knockout Techniques
Human Embryonic Stem Cells*
Humans

Substances

Cell Cycle Proteins
GADD45A protein, human