Simultaneous detection and speciation of mono- and di-valent copper ions with a dual-channel fluorescent nanoprobe

An-Qi Zheng; Chen-Xi Zhao; Xiao-Juan Wang; Yang Shu; Jian-Hua Wang

doi:10.1039/d0cc06750d

Simultaneous detection and speciation of mono- and di-valent copper ions with a dual-channel fluorescent nanoprobe

Chem Commun (Camb). 2020 Dec 18;56(97):15337-15340. doi: 10.1039/d0cc06750d. Epub 2020 Nov 24.

Authors

An-Qi Zheng¹, Chen-Xi Zhao, Xiao-Juan Wang, Yang Shu, Jian-Hua Wang

Affiliation

¹ Research Center for Analytical Sciences, Department of Chemistry, Northeastern University, Box 332, Shenyang 110819, China. shuyang@mail.neu.edu.cn jianhuajrz@mail.neu.edu.cn.

PMID: 33231237
DOI: 10.1039/d0cc06750d

Abstract

Redox cyclings between mono-/di-valent copper oxidation states occur in electron transfer reactions that underlie their biological functions. We report herein a dual channel fluorescent nanoprobe for the detection of mono- and di-valent copper ions. The probe, BSA-CDs@RBH/BCS, is designed by decorating carbon dots (CDs) on BSA encapsulated rhodamine hydrazide (RBH) and conjugating with bathocuproine disulfonate (BCS). Cu²⁺ catalyzes the hydrolysis of RBH, and the formed rhodamine B (RhB) shows emission at λ_ex/λ_em = 360/575 nm which ensures Cu²⁺ detection. BSA reduces Cu²⁺ to Cu⁺ and the BCS-Cu⁺ chelate shows emission at λ_ex/λ_em 360/450 nm which ensures Cu⁺ assay. Thus, the dual-channel fluorescence enables speciation of Cu²⁺ and Cu⁺.