Dataset of antibody variable region sequence features inferred from a respiratory syncytial virus fusion protein-specific B cell receptor repertoire induced by natural infection of a healthy adult

Gerald Schneikart; Simona Tavarini; Chiara Sammicheli; Giulia Torricelli; Silvia Guidotti; Ugo D'Oro; Oretta Finco; Monia Bardelli

doi:10.1016/j.dib.2020.106499

Dataset of antibody variable region sequence features inferred from a respiratory syncytial virus fusion protein-specific B cell receptor repertoire induced by natural infection of a healthy adult

Data Brief. 2020 Nov 4:33:106499. doi: 10.1016/j.dib.2020.106499. eCollection 2020 Dec.

Authors

Gerald Schneikart^{1

2}, Simona Tavarini¹, Chiara Sammicheli¹, Giulia Torricelli¹, Silvia Guidotti¹, Ugo D'Oro¹, Oretta Finco¹, Monia Bardelli¹

Affiliations

¹ GSK, Via Fiorentina 1, 53100 Siena, Italy.
² Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.

Abstract

Respiratory syncytial virus (RSV) is the primary cause for acute lower respiratory syndrome in children younger than 5 years. Research on B cell repertoires and antibodies binding the RSV fusion protein (RSV F) is of major interest in the development of potential vaccine candidates and therapies. B cell receptors (BCRs) which have higher affinities for a specific antigen are preferentially selected for B cell clonal expansion in germinal center reactions. Consequently, antigen-specific BCR repertoires share common features, as for instance preferential variable gene usage, variable region mutation levels or lengths of the heavy chain complementarity-determining region 3. Since RSV repeatedly infects every person throughout life, memory B cells (MBC) expressing RSV F-binding BCRs circulate in the blood of healthy adults. This dataset of BCR variable region sequence features was derived from single cell-sorted RSV F-directed MBCs of a healthy adult blood donor [1]. The dataset was produced with publicly available data analysis software programs and scripts, which facilitates integration or comparison with antibody sequence repertoire data of different individuals derived with the same or comparable data analysis approaches and tools.

Keywords: Antibody repertoire analysis; B cell receptor repertoire; Clonal expansion; Clonal relatedness; Fusion protein; Memory B cell; Natural infection; Respiratory syncytial virus.