Hydrolysis and Methanogenesis in UASB-AnMBR Treating Municipal Wastewater Under Psychrophilic Conditions: Importance of Reactor Configuration and Inoculum

Judit Ribera-Pi; Antonio Campitelli; Marina Badia-Fabregat; Irene Jubany; Xavier Martínez-Lladó; Ewan McAdam; Bruce Jefferson; Ana Soares

doi:10.3389/fbioe.2020.567695

Hydrolysis and Methanogenesis in UASB-AnMBR Treating Municipal Wastewater Under Psychrophilic Conditions: Importance of Reactor Configuration and Inoculum

Front Bioeng Biotechnol. 2020 Nov 2:8:567695. doi: 10.3389/fbioe.2020.567695. eCollection 2020.

Authors

Judit Ribera-Pi¹, Antonio Campitelli², Marina Badia-Fabregat¹, Irene Jubany¹, Xavier Martínez-Lladó¹, Ewan McAdam², Bruce Jefferson², Ana Soares²

Affiliations

¹ Eurecat, Centre Tecnològic de Catalunya, Water, Air and Soil Unit, Manresa, Spain.
² Cranfield Water Science Institute, Vincent Building, Cranfield University, Cranfield, United Kingdom.

Abstract

Three upflow anaerobic sludge blanket (UASB) pilot scale reactors with different configurations and inocula: flocculent biomass (F-UASB), flocculent biomass and membrane solids separation (F-AnMBR) and granular biomass and membrane solids separation (G-AnMBR) were operated to compare start-up, solids hydrolysis and effluent quality. The parallel operation of UASBs with these different configurations at low temperatures (9.7 ± 2.4°C) and the low COD content (sCOD 54.1 ± 10.3 mg/L and pCOD 84.1 ± 48.5 mg/L), was novel and not previously reported. A quick start-up was observed for the three reactors and could be attributed to the previous acclimation of the seed sludge to the settled wastewater and to low temperatures. The results obtained for the first 45 days of operation showed that solids management was critical to reach a high effluent quality. Overall, the F-AnMBR showed higher rates of hydrolysis per solid removed (38%) among the three different UASB configurations tested. Flocculent biomass promoted slightly higher hydrolysis than granular biomass. The effluent quality obtained in the F-AnMBR was 38.0 ± 5.9 mg pCOD/L, 0.4 ± 0.9 mg sCOD/L, 9.9 ± 1.3 mg BOD₅/L and <1 mg TSS/L. The microbial diversity of the biomass was also assessed. Bacteroidales and Clostridiales were the major bacterial fermenter orders detected and a relative high abundance of syntrophic bacteria was also detected. Additionally, an elevated abundance of sulfate reducing bacteria (SRB) was also identified and was attributed to the low COD/SO₄ ^2- ratio of the wastewater (0.5). Also, the coexistence of acetoclastic and hydrogenotrophic methanogenesis was suggested. Overall this study demonstrates the suitability of UASB reactors coupled with membrane can achieve a high effluent quality when treating municipal wastewater under psychrophilic temperatures with F-AnMBR promoting slightly higher hydrolysis rates.

Keywords: anaerobic membrane bioreactor; hydrolysis; microbial community; municipal wastewater; psychrophilic temperature; upflow anaerobic sludge blanket.