Two-stage enrichment of hydrogen-oxidizing bacteria as biofertilizers

Wei Zhang; Yong-Xin Li; Yun Niu; Fang Zhang; Yi-Bing Li; Raymond Jianxiong Zeng

doi:10.1016/j.chemosphere.2020.128932

Two-stage enrichment of hydrogen-oxidizing bacteria as biofertilizers

Chemosphere. 2021 Mar:266:128932. doi: 10.1016/j.chemosphere.2020.128932. Epub 2020 Nov 10.

Authors

Wei Zhang¹, Yong-Xin Li², Yun Niu², Fang Zhang², Yi-Bing Li², Raymond Jianxiong Zeng³

Affiliations

¹ Centre of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; CAS Key Laboratory for Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei, 230026, China.
² Centre of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
³ Centre of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; CAS Key Laboratory for Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei, 230026, China. Electronic address: rzeng@ustc.edu.cn.

PMID: 33220977
DOI: 10.1016/j.chemosphere.2020.128932

Abstract

Biofertilizers can replace chemical fertilizers to promote the plant growth without causing any pollution. The study of hydrogen-oxidizing bacteria (HOB) enrichment as biofertilizers from mixed culture is scarce. Our recent study shows that biofertilizing HOB are successfully enriched in a short hydraulic retention time of 10 h. While, the mechanism is unknown. This study intentionally used a two-stage method to enrich biofertilizing HOB specifically with nitrate as nitrogen source in Stage 1 and then 1-aminocyclopropane-1-carboxylate (ACC) as nitrogen source in Stage 2. It was found Pseudomonas (34.46%, reported HOB) predominated in Stage 1, while Azospirillum (59.35%), Azoarcus (36%) were dominant genera and Azospirillum lipoferum strain DSM 1691 (50%), Azoarcus olearius strain DQS-4 (50%) were dominant species in Stage 2. The enriched HOB of Stage 2 showed ACC deaminase activity. Furthermore, they could also fix N₂ and consume Ca₃(PO₄)₂. Thus, the two-stage method can be used as a specific enrichment for HOB as biofertilizers, which extends the application of HOB in agriculture.

Keywords: 1-Aminocyclopropane-1-carboxylate (ACC); Biofertilizers; Hydrogen-oxidizing bacteria; Mixed culture; Two-stage enrichment.

MeSH terms

Azoarcus / metabolism
Bacteria* / metabolism
Carbon-Carbon Lyases / metabolism
Hydrogen*
Oxidation-Reduction

Substances

Hydrogen
Carbon-Carbon Lyases

Supplementary concepts

Azoarcus olearius