Comparison of nitrite accumulation performance and microbial community structure in endogenous partial denitrification process with acetate and glucose served as carbon source

Abstract

Although the combination of endogenous partial denitrification (EPD) and Anammox (EPD-AMX) were developed for deep-level nitrogen removal, the effects of different carbon source were not clear. In this study, the EPD performance was investigated comparatively with acetate (EPD_A) and glucose (EPD_G). Results revealed that through regulating chemical oxygen demand to phosphate ratio, Candidatus_Competibacter was highly enriched in EPD_A (54.2%) and EPD_G (51.3%), resulting high intracellular carbon storage efficiencies (90.2% and 85.3%, respectively). More stable nitrite accumulation was observed in EPD_G than EPD_A. But, higher specific nitrite generated rate (r_NO2, 8.25 > 7.04 mgN·gVSS^-1·h^-1) and nitrate-to-nitrite transformation rate (NTR, 87.9% > 85.2%) were achieved in EPD_A than those in EPD_G. The functional bacterium was also shifted to Defluviicoccus in both EPD_A (30.6%) and EPD_G (25.8%). Moreover, with whether acetate or glucose, the EPD-AMX processes could achieve the same level of total nitrogen removal efficiencies (88.7% and 91.3%, respectively) via anammox mainly (87.8% and 89.4%, respectively).

Keywords: Anammox; Biological nitrogen removal (BNR); Carbon source; Denitrifying glycogen accumulating organisms (DGAOs); Endogenous partial denitrification (EPD).