We detected 3 lyssaviruses in insectivorous bats sampled in South Africa during 2003-2018. We used phylogenetic analysis to identify Duvenhage lyssavirus and a potentially new lyssavirus, provisionally named Matlo bat lyssavirus, that is related to West Caucasian bat virus. These new detections highlight that much about lyssaviruses remains unknown.
Keywords: Duvenhage virus; Matlo bat lyssavirus; South Africa; West Caucasian bat virus; bats; encephalitis; insectivorous bats; lyssaviruses; rabies; surveillance; viral zoonoses; viruses; zoonoses.