Characterization of a Plasmid-Encoded Resistance-Nodulation-Division Efflux Pump in Klebsiella pneumoniae and Klebsiella quasipneumoniae from Patients in China

Ruowen He; Yongqiang Yang; Yiping Wu; Lan-Lan Zhong; Yanxian Yang; Guanping Chen; Mingyang Qin; Xiaoxue Liang; Mohamed Abd El-Gawad El-Sayed Ahmed; Minmin Lin; Bin Yan; Yong Xia; Min Dai; Hongtao Chen; Guo-Bao Tian

doi:10.1128/AAC.02075-20

Characterization of a Plasmid-Encoded Resistance-Nodulation-Division Efflux Pump in Klebsiella pneumoniae and Klebsiella quasipneumoniae from Patients in China

Antimicrob Agents Chemother. 2021 Jan 20;65(2):e02075-20. doi: 10.1128/AAC.02075-20. Print 2021 Jan 20.

Authors

Ruowen He^#^{1

2}, Yongqiang Yang^#^{1

2

3}, Yiping Wu^#^{1

2}, Lan-Lan Zhong^#^{1

2}, Yanxian Yang^{1

2}, Guanping Chen⁴, Mingyang Qin⁵, Xiaoxue Liang⁶, Mohamed Abd El-Gawad El-Sayed Ahmed^{1

2

7}, Minmin Lin⁸, Bin Yan⁹, Yong Xia¹⁰, Min Dai^#¹¹, Hongtao Chen^#¹², Guo-Bao Tian^#^{13

2

14}

Affiliations

¹ Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
² Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, China.
³ School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou, China.
⁴ Sun Yat-sen University School of Medicine, Guangzhou, China.
⁵ Basic Medical College, Xinxiang Medical University, Xinxiang, China.
⁶ School of Laboratory Medicine, Chengdu Medical College, Chengdu, China.
⁷ Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, Cairo, 6th of October City, Egypt.
⁸ Department of Respiratory Medicine, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.
⁹ Department of Neonatal Surgery, Guangzhou Women and Children's Medical Center, Guangzhou, China.
¹⁰ Department of Clinical Laboratory Medicine, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
¹¹ School of Laboratory Medicine, Chengdu Medical College, Chengdu, China daimin1015@163.com 13926925941@139.com tiangb@mail.sysu.edu.cn.
¹² Department of Clinical Lab, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, China daimin1015@163.com 13926925941@139.com tiangb@mail.sysu.edu.cn.
¹³ Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China daimin1015@163.com 13926925941@139.com tiangb@mail.sysu.edu.cn.
¹⁴ School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China.

^# Contributed equally.

Abstract

Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1 The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.

Keywords: Klebsiella spp.; antimicrobial resistance; efflux pump; tigecycline; tmexCD1-toprJ1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
China
Humans
Klebsiella
Klebsiella Infections* / drug therapy
Klebsiella pneumoniae* / genetics
Microbial Sensitivity Tests
Plasmids / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins

Supplementary concepts

Klebsiella quasipneumoniae