Bone Marrow Stromal Cell Assays: In Vitro and In Vivo

Pamela G Robey; Sergei A Kuznetsov; Paolo Bianco; Mara Riminucci

doi:10.1007/978-1-0716-1028-2_23

Bone Marrow Stromal Cell Assays: In Vitro and In Vivo

Methods Mol Biol. 2021:2230:379-396. doi: 10.1007/978-1-0716-1028-2_23.

Authors

Pamela G Robey¹, Sergei A Kuznetsov², Paolo Bianco³, Mara Riminucci³

Affiliations

¹ Skeletal Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA. probey@dir.nidcr.nih.gov.
² Skeletal Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
³ Sapienza University of Rome, Rome, Italy.

PMID: 33197027
DOI: 10.1007/978-1-0716-1028-2_23

Abstract

Populations of bone marrow stromal cells (BMSCs, also known as bone marrow-derived "mesenchymal stem cells") contain a subset of cells that are able to recapitulate the formation of a bone/marrow organ (skeletal stem cells, SSCs). It is now apparent that cells with similar but not identical properties can be isolated from other skeletal compartments (growth plate, periosteum). The biological properties of BMSCs, and these related stem/progenitor cells, are assessed by a variety of assays, both in vitro and in vivo. Application of these assays in an appropriate fashion provide a great deal of information on the role of BMSCs, and the subset of SSCs, in health and in disease.

Keywords: Bone; Bone marrow; Cartilage; Colony forming unit-fibroblast; In vitro assays; In vivo transplantation; Marrow adipocytes; Stroma.

Publication types

Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow Cells / ultrastructure*
Bone and Bones / ultrastructure*
Cell Differentiation / genetics
Colony-Forming Units Assay / methods*
Growth Plate / ultrastructure
Humans
Mesenchymal Stem Cells / ultrastructure*