Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications

Christopher J Rowlands; Oliver T Bruns; Daniel Franke; Dai Fukamura; Rakesh K Jain; Moungi G Bawendi; Peter T C So

doi:10.1088/1361-6463/ab16b4

Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications

J Phys D Appl Phys. 2019 Jun 26;52(26):264001. doi: 10.1088/1361-6463/ab16b4. Epub 2019 Apr 25.

Authors

Christopher J Rowlands^{1

2}, Oliver T Bruns³, Daniel Franke⁴, Dai Fukamura⁵, Rakesh K Jain^{5

6}, Moungi G Bawendi⁴, Peter T C So^{7

8}

Affiliations

¹ Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom.
² c.rowlands@imperial.ac.uk.
³ Helmholtz Pioneer Campus (HPC), Helmholtz Zentrum München, 85764 Neuherberg, Germany.
⁴ Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, United States of America.
⁵ Edwin L. Steele Laboratory for Tumour Biology, Massachusetts General Hospital, Boston, MA, United States of America.
⁶ Harvard Medical School, Cambridge, MA, United States of America.
⁷ Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States of America.
⁸ Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States of America.

Abstract

The first ever demonstration of temporal focusing with short wave infrared (SWIR) excitation and emission is demonstrated, achieving a penetration depth of 500 µm in brain tissue. This is substantially deeper than the highest previously-reported values for temporal focusing imaging in brain tissue, and demonstrates the value of these optimized wavelengths for neurobiological applications.

Keywords: fluorescence microscopy; multiphoton microscopy; neurophotonics; quantum dots; temporal focusing.

Abstract

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