Sfrp5/Wnt5a and leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease

Biyu Wang; Yu Pan; Guang Yang; Zong'ao Cui; Wenping Yu; Hui Liu; Bing Bai

doi:10.1016/j.clinbiochem.2020.11.002

Sfrp5/Wnt5a and leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease

Clin Biochem. 2021 Jan:87:46-51. doi: 10.1016/j.clinbiochem.2020.11.002. Epub 2020 Nov 12.

Authors

Biyu Wang¹, Yu Pan², Guang Yang¹, Zong'ao Cui¹, Wenping Yu¹, Hui Liu¹, Bing Bai³

Affiliations

¹ The Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
² The Department of Medical Administration, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
³ The Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: baibingbaibing2020@163.com.

PMID: 33188773
DOI: 10.1016/j.clinbiochem.2020.11.002

Abstract

Background: Leptin, adiponectin, secreted frizzled-related protein 5 (Sfrp5) and wingless-type family member 5a (Wnt5a) are novel adipokines that are involved in insulin sensitivity and atherosclerosis. The aim of the present study was to investigate the serum and periarterial adipose tissue leptin/adiponectin and Sfrp5/Wnt5a levels in patients with peripheral arterial occlusive disease (PAOD).

Methods: A total of 75 patients with PAOD and 39 control subjects were recruited. The serum concentrations of leptin, adiponectin, Sfrp5 and Wnt5a were measured by ELISAs, and the leptin, adiponectin, Sfrp5 and Wnt5a levels in the periarterial adipose tissue were observed by western blotting.

Results: The serum Sfrp5 levels were significantly lower in the patients with PAOD than in the control subjects (p < 0.001) and Wnt5a levels were higher in the patients with PAOD (p < 0.001). The serum leptin levels were significantly higher in the patients with PAOD than in the control subjects (p < 0.001), and adiponectin levels were significantly lower in the patients with PAOD (p < 0.001). The serum Sfrp5 levels were associated with ABI (rs = 0.274; p = 0.018), Wnt5a (rs = -0.409; p < 0.001), adiponectin (rs = 0.244; p = 0.035) and Leptin/Adiponetin ratio (rs = -0.244; p = 0.037). The adiponectin and Sfrp5 protein levels were decreased in the periarterial adipose tissue of patients with PAOD compared with control subjects. The leptin and Wnt5a protein levels were increased in the periarterial adipose tissue of patients with PAOD compared with control subjects.

Conclusion: We demonstrated that the adiponectin and Sfrp5 levels in the serum and periarterial adipose tissue were significantly lower in the patients with PAOD than in the control subjects. The leptin and Wnt5a levels in the serum and periarterial adipose tissue were significantly higher in the patients with PAOD than in the control subjects.

Keywords: Adiponectin; Ankle-brachial index; Periarterial adipose tissue; Peripheral arterial occlusive disease; Sfrp5.

MeSH terms

Adaptor Proteins, Signal Transducing / blood*
Adiponectin / blood*
Adipose Tissue / metabolism*
Adipose Tissue / pathology
Aged
Arterial Occlusive Diseases / blood
Arterial Occlusive Diseases / pathology*
Biomarkers / blood
Body Mass Index
Case-Control Studies
Female
Humans
Insulin Resistance
Leptin / blood*
Male
Peripheral Arterial Disease / blood
Peripheral Arterial Disease / pathology*
Wnt-5a Protein / blood*

Substances

ADIPOQ protein, human
Adaptor Proteins, Signal Transducing
Adiponectin
Biomarkers
LEP protein, human
Leptin
SFRP5 protein, human
WNT5A protein, human
Wnt-5a Protein

Supplementary concepts

Peripheral Arterial Occlusive Disease 1