Distribution Characteristics of ANA and ANCA in Patients with Hyperthyroidism

Abstract

Background: Antinuclear antibody (ANA) and Antineutrophil autoantibodies (ANCA) are often used as markers for the diagnosis of autoimmune diseases. In clinical practice, we have found that ANA and ANCA often occur in sera of patients with hyperthyroidism.

Objective: The study aimed to discover the positive features of anti-nuclear antibodies (ANA) and anti-neutrophil cytoplasmic antibodies (ANCA) in hyperthyroidism.

Materials and methods: In sera samples from 171 patients with hyperthyroidism and 50 healthy controls, ANA and ANCA were detected with indirect immunofluorescence (IIF) method.

Results: ANA and ANCA positive rates were higher in hyperthyroidism (33.9% and 29.2%, respectively) than those in control (4.0% and 2.0%, respectively); the low titer (≤1:320)of ANA was dominated (86.2%) in 58 hyperthyroidism patients with positive ANA, the major pattern involved homogeneous (48.3%) and speckled patterns (48.3%). The low titer (≤1:32) of ANCA was dominated( 64.6%) in 50ANCA positive samples and the major pattern was perinuclear (p) ANCA (96%).

Conclusion: The positive rates of ANA and ANCA were increased and mainly appeared of low titer in hyperthyroidism, which supplement the laboratory characteristics of hyperthyroidism.

Keywords: ANA; ANCA; Immunofluorescence; autoantibody.; distribution characteristics; hyperthyroidism.