S-adenosyl methionine synthetase SAMS-5 mediates dietary restriction-induced longevity in Caenorhabditis elegans

PLoS One. 2020 Nov 11;15(11):e0241455. doi: 10.1371/journal.pone.0241455. eCollection 2020.

Abstract

S-adenosyl methionine synthetase (SAMS) catalyzes the biosynthesis of S-adenosyl methionine (SAM), which serves as a universal methyl group donor for numerous biochemical reactions. Previous studies have clearly demonstrated that SAMS-1, a C. elegans homolog of mammalian SAMS, is critical for dietary restriction (DR)-induced longevity in Caenorhabditis elegans. In addition to SAMS-1, three other SAMS paralogs have been identified in C. elegans. However, their roles in longevity regulation have never been explored. Here, we show that depletion of sams-5, but not sams-3 or sams-4, can extend lifespan in worms. However, the phenotypes and expression pattern of sams-5 are distinct from sams-1, suggesting that these two SAMSs might regulate DR-induced longevity via different mechanisms. Through the genetic epistasis analysis, we have identified that sams-5 is required for DR-induced longevity in a pha-4/FOXA dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / enzymology*
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Caloric Restriction*
  • Gene Expression Regulation
  • Larva / genetics
  • Longevity / physiology*
  • Methionine Adenosyltransferase / metabolism*
  • Mutation / genetics
  • Phenotype
  • Transcription Factors / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Transcription Factors
  • Methionine Adenosyltransferase

Grants and funding

The authors received funding from the Minister of Science and Technology, Taiwan with the grants NSC 103-2311-B-010-007 and MOST 106-2311-B-010-005 to T.C. and MOST 108-2628-B-010-002 to A.H.. T.C. was also funded by National Health Research Institute, Taiwan (NHRI-EX103-1034NI). Some strains were provided by the Caenorhabditis Genetics Center (CGC). Taiwan C. elegans Core Facility (CECF) provided technical support. The funders had no role in study design, data collection and analysis to publish, or preparation of the manuscript.