DEC-205 receptor-mediated long-circling nanoliposome as an antigen and Eucommia ulmoides polysaccharide delivery system enhances the immune response via facilitating dendritic cells maturation

Haibo Feng; Xiaonong Yang; Jing Fan; Linzi Zhang; Qianqian Liu; Dongkun Chai

doi:10.1080/10717544.2020.1844343

DEC-205 receptor-mediated long-circling nanoliposome as an antigen and Eucommia ulmoides polysaccharide delivery system enhances the immune response via facilitating dendritic cells maturation

Drug Deliv. 2020 Dec;27(1):1581-1596. doi: 10.1080/10717544.2020.1844343.

Authors

Haibo Feng^{1

2}, Xiaonong Yang^{1

2}, Jing Fan³, Linzi Zhang^{1

2}, Qianqian Liu^{1

2}, Dongkun Chai⁴

Affiliations

¹ College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu, P. R. China.
² Key Laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, P. R. China.
³ Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, P. R. China.
⁴ Department of Veterinary Medicine, Southwest University, Rongchang, P. R. China.

Abstract

DEC-205 receptor-mediated dendritic cells (DC) targeting nanoliposomes is a promising delivery system in eliciting an immune response against pathogens. When this delivery system carries both antigen and immunomodulator, it can effectively regulate the DC function as well as the initial T cell response. To maximize the desired therapeutic effects of Eucommia ulmoides Oliv. polysaccharides (EUPS), and induce an efficient humoral and cellular immune response against an antigen, we encapsulated the OVA and EUPS in long-circling nanoliposomes and conjugated it with anti-DEC-205 receptor antibody to obtain a DEC-205-targeted nanoliposomes (anti-DEC-205-EUPS-OVA-LPSM). The physicochemical properties and immune-modulating effects were investigated in vitro and in vivo by a series of the experiment to evaluate the targeting efficiency of anti-DEC-205-EUPS-OVA-LPSM. In vitro, anti-DEC-205-EUPS-OVA-LPSM (160 μg mL^-1) could enhance DCs proliferation and increase their phagocytic efficiency. In vivo anti-DEC-205-EUPS-OVA-LPSM remarkably promoted the OVA-specific IgG and IgG isotypes levels, enhanced the splenocyte proliferation, and induced the NK cell and CTL cytotoxicity. Besides, the anti-DEC-205-EUPS-OVA-LPSM enhanced the maturation of DCs. These findings suggest that the DEC-205 receptor antibody-conjugated EUPS nanoliposome can act as an efficient antigen delivery system to enhance the cellular and humoral immune response by promoting DC maturation. This indicates that the anti-DEC-205-EUPS-OVA-LPSM has significant potential as an immune-enhancing agent and antigen delivery system.

Keywords: Eucommia ulmoides polysaccharide; DEC-205 receptor; dendritic cells; immune response; nanoliposome.

MeSH terms

Animals
Antigens / administration & dosage*
Antigens, CD / metabolism*
Cells, Cultured
Dendritic Cells / drug effects*
Eucommiaceae / chemistry*
Female
Immunity / drug effects*
Immunologic Factors / immunology
Lectins, C-Type / metabolism*
Liposomes / administration & dosage*
Mice
Mice, Inbred ICR
Minor Histocompatibility Antigens / metabolism*
Nanoparticles / administration & dosage*
Polysaccharides / administration & dosage*
Receptors, Cell Surface / metabolism*

Substances

Antigens
Antigens, CD
DEC-205 receptor
Immunologic Factors
Lectins, C-Type
Liposomes
Minor Histocompatibility Antigens
Polysaccharides
Receptors, Cell Surface

Grants and funding

The project was financially supported by the National Natural Science Foundation of China (Project No. 31872511) and in part by the Fundamental Research Funds for the Central Universities (Project No. 2020NYB22).