Establishment of non-integrate induced pluripotent stem cell line CSUASOi006-A, from urine-derived cells of a PRPF8-related dominant retinitis pigmentosa patient

Yalan Zhou; Zekai Cui; Yutong Jing; Shengru Mao; Donglong Chen; Chengcheng Ding; Jianing Gu; Hon Fai Chan; Shibo Tang; Jiansu Chen

doi:10.1016/j.scr.2020.102041

Establishment of non-integrate induced pluripotent stem cell line CSUASOi006-A, from urine-derived cells of a PRPF8-related dominant retinitis pigmentosa patient

Stem Cell Res. 2020 Dec:49:102041. doi: 10.1016/j.scr.2020.102041. Epub 2020 Oct 12.

Authors

Yalan Zhou¹, Zekai Cui², Yutong Jing³, Shengru Mao⁴, Donglong Chen⁵, Chengcheng Ding⁶, Jianing Gu⁷, Hon Fai Chan⁸, Shibo Tang⁹, Jiansu Chen¹⁰

Affiliations

¹ Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: 641852254@qq.com.
² Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: cuizk@qq.com.
³ Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: 13572560975@163.com.
⁴ Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: 307405780@qq.com.
⁵ Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: cdlong713@hotmail.com.
⁶ Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: d_cheng55@163.com.
⁷ Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China. Electronic address: jennie.gu@163.com.
⁸ Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, Hong Kong, PR China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, PR China. Electronic address: honfaichan@cuhk.edu.hk.
⁹ Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai 200031, PR China. Electronic address: tangsb@mail.sysu.edu.com.
¹⁰ Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, PR China. Electronic address: chenjiansu2000@163.com.

PMID: 33157387
DOI: 10.1016/j.scr.2020.102041

Abstract

Retinitis pigmentosa (RP) is a group of inherited retinal disorders characterized by the progressive photoreceptors and pigment epithelial cells dysfunction. Here, we report the human induced pluripotent stem cell line (iPSC) CSUASOi006-A, generated from urine-derived cells (UCs) of a 17-year-old male patient with clinically diagnosed RP carrying point mutation (c.C5792T) in the pre-mRNA processing factor 8 gene (PRPF8). The newly derived CSUASOi006-A cell line has the patient's same mutation (c.C5792T) and could provide useful resources for studying the pathogenic mechanism of PRPF8-related RP.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Humans
Induced Pluripotent Stem Cells*
Male
Mutation
Point Mutation
RNA-Binding Proteins / genetics
Retinitis Pigmentosa* / genetics

Substances

PRPF8 protein, human
RNA-Binding Proteins