Effects of Saccharomyces cerevisiae and phytase co-fermentation of wheat bran on growth, antioxidation, immunity and intestinal morphology in broilers

Wen-Yang Chuang; Li-Jen Lin; Yun-Chen Hsieh; Shen-Chang Chang; Tzu-Tai Lee

doi:10.5713/ajas.20.0399

Effects of Saccharomyces cerevisiae and phytase co-fermentation of wheat bran on growth, antioxidation, immunity and intestinal morphology in broilers

Anim Biosci. 2021 Jul;34(7):1157-1168. doi: 10.5713/ajas.20.0399. Epub 2020 Oct 13.

Authors

Wen-Yang Chuang¹, Li-Jen Lin², Yun-Chen Hsieh¹, Shen-Chang Chang³, Tzu-Tai Lee^{1

4}

Affiliations

¹ Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan.
² School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 402, Taiwan.
³ Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, 912, Taiwan.
⁴ The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, 402, Taiwan.

Abstract

Objective: The aim of this study was to investigate the effects of different amounts of wheat bran (WB) inclusion and postbiotics form by Saccharomyces cerevisiae and phytase cofermented wheat bran (FWB) on the growth performance and health status of broilers.

Methods: Study randomly allocated a total of 300 male broilers to a control and 4 treatment groups (5% WB, 5% FWB, 10% WB, and 10% FWB inclusion, respectively) with each pen having 20 broilers and 3 pens per treatment.

Results: The WB does not contain enzymes, but there are 152.8, 549.2, 289.5, and 147.1 U/g dry matter xylanase, protease, cellulase and β-glucanase in FWB, respectively. Furthermore, FWB can decrease nitric oxide release of lipopolysaccharide stimulated chicken peripheral blood mononuclear cells by about two times. Results show that 10% FWB inclusion had significantly the highest weight gain (WG) at 1 to 21 d; 5% FWB had the lowest feed conversion rate at 22 to 35 d; 10% WB and 10% FWB inclusion have the highest villus height and Lactobacillus spp. number in caecum; and both 5% and 10% FWB can increase ash content in femurs. Compared to control group, all treatments increase mucin 2, and tight junction (TJ), such as occludin, claudin-1, zonula occludens-1, and mRNA expression in ileum by at least 5 folds. In chicken peripheral blood mononuclear cells, nicotinamide adenine dinucleotide phosphate-oxidase-1 mRNA expression decreases from 2 to 5 times, and glutamate-cysteine ligase catalytic subunit mRNA expression also increases in all treatment groups compared to control group. The mRNA expression of pro-inflammatory cytokines, including interleukin-6 (IL-6), nuclear factor-κB, and IL-1β, decreases in 5% and 10% FWB groups compared to control group.

Conclusion: To summarize, both WB and FWB inclusion in broilers diets increase TJ mRNA expression and anti-oxidation and anti-inflammation, but up to 10% FWB groups have better WG in different stages of broiler development.

Keywords: Co-fermented; Phytase; Postbiotic; Saccharomyces cerevisiae; Wheat Bran.

Abstract

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