Endogenous Labeling for Light Microscopy during HIV-1 Immune Responses

Minette Salmon; Irene Carlon-Andres; Sergi Padilla-Parra

doi:10.1016/j.it.2020.10.009

Endogenous Labeling for Light Microscopy during HIV-1 Immune Responses

Trends Immunol. 2020 Dec;41(12):1056-1059. doi: 10.1016/j.it.2020.10.009. Epub 2020 Nov 2.

Authors

Minette Salmon¹, Irene Carlon-Andres¹, Sergi Padilla-Parra²

Affiliations

¹ Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.
² Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK; Department of Infectious Diseases, King's College London, Faculty of Life Sciences and Medicine, London SE1 9RT, UK; Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 9RT, UK. Electronic address: sergio.padilla_parra@kcl.ac.uk.

PMID: 33148466
DOI: 10.1016/j.it.2020.10.009

Abstract

New approaches in single molecule spectroscopy and microscopy are able to resolve the spatial and temporal resolution of T cell receptor signaling in the context of immune responses to HIV-1 infection. These approaches need to be complemented with novel techniques that endogenously tag the protein or proteins of interest, yet avoid overexpression, to image protein dynamics under physiological conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HIV-1* / immunology
Humans
Immunity* / immunology
Microscopy* / trends
Proteins / chemistry
Receptors, Antigen, T-Cell / metabolism
Signal Transduction / physiology
Staining and Labeling* / methods
Staining and Labeling* / trends

Substances

Proteins
Receptors, Antigen, T-Cell

Grants and funding

203141/WT_/Wellcome Trust/United Kingdom