Detection of galactooligosaccharides with high lactose interference in infant formula using a simple single epimer chromatography

Minjie Zhang; Lin Luo; Songbai Liu; Haoxin Hu; Riming Huang; Yuanming Sun; Hongtao Lei; Xiaoqun Wei

doi:10.1016/j.foodchem.2020.128367

Detection of galactooligosaccharides with high lactose interference in infant formula using a simple single epimer chromatography

Food Chem. 2021 Apr 16:342:128367. doi: 10.1016/j.foodchem.2020.128367. Epub 2020 Oct 14.

Authors

Minjie Zhang¹, Lin Luo², Songbai Liu³, Haoxin Hu⁴, Riming Huang⁵, Yuanming Sun⁶, Hongtao Lei⁷, Xiaoqun Wei⁸

Affiliations

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: zhangminjie@stu.scau.edu.cn.
² Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: lin.luo@scau.edu.cn.
³ Wen's Foodstuffs Group Co., Ltd, Guangdong Province, Xinxing, 527400, China.
⁴ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: 13025264196@stu.scau.edu.cn.
⁵ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: huangriming@scau.edu.cn.
⁶ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: ymsun@scau.edu.cn.
⁷ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: hongtao@scau.edu.cn.
⁸ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: weixqun@scau.edu.cn.

PMID: 33148428
DOI: 10.1016/j.foodchem.2020.128367

Abstract

Robust and more anti-interference enzymatic quantification of galactooligosaccharide (GOS) is important in consumer protection. However, many methods with harsh conditions could hardly separate GOS's hydrolysates galactose from its structurally similar isomer glucose, since each of them has double epimers, especially to determinate a trace of GOS from large amounts of lactose in the food matrix. The investigation was designed to solve the problem by using High Performance Liquid Chromatography with Evaporative Light Scattering Detector (HPLC-ELSD), applying friendly mobile phase and column. Result showed the content of galactose was seldom affected even by a high content of glucose by integrating the peak area of an excellent resolution single epimer. Moreover, the method existed a good linearity and stability (recovery rate at 90.5-105.1%), which met the statutory limit requirement for the quantitative analysis of concentrated GOS in infant formula. It was also helpful for separating and quantifying other sugar or epimers.

Keywords: Galactooligosaccharides; High performance liquid chromatography; Infant formulas; Quantification.

MeSH terms

Chromatography, High Pressure Liquid
Food Analysis / methods*
Galactose / chemistry*
Humans
Infant
Infant Formula / chemistry*
Lactose / analysis*
Oligosaccharides / analysis*
Oligosaccharides / chemistry*
Stereoisomerism

Substances

Oligosaccharides
Lactose
Galactose