Croceivirga litoralis sp. nov., isolated from coastal surface water, and reclassification of Muricauda lutea as Croceivirga lutea comb. nov

Abstract

A novel Gram-stain-negative, aerobic, yellow-pigmented bacterium was isolated from seawater of Aoshan Bay, and designated as strain ASW18^T. Strain ASW18^T was a long-rod-shaped bacterium without flagellum and lacked gliding ability. Based on 16S rRNA gene phylogeny, strain ASW18^T showed the closest relationship to Croceivirga radicis MCCC 1A06690^T, with a sequence similarity of 97.0 %. Strain ASW18^T was able to grow at 25-40 °C, at pH 5.5-9.5 and with 0.5-9 % (w/v) NaCl. The genomic DNA G+C content of strain ASW18^T was 37.3 %. The predominant cellular fatty acids of strain ASW18^T were iso-C_{15 : 0}, iso-C_{17 : 0} 3-OH and iso-C_{15 : 1} G. The major polar lipids were phosphatidylethanolamine, phosphatidyldimethylethanolamine, an aminolipid and three unidentified lipids. The respiratory quinone of strain ASW18^T was menaquinone with six isoprene units (MK-6). Based on the present polyphasic analysis, strain ASW18^T represents a novel species of the genus Croceivirga, for which the name Croceivirga litoralis sp. nov. is proposed; the type strain is ASW18^T (=MCCC 1K04203^T=KCTC 72852^T). In addition, it is also proposed that Muricauda lutea should be reclassified as Croceivirga lutea comb. nov.; the type strain is CSW06^T (=CGMCC 1.15761^T=JCM 31455^T=KCTC 52375^T=MCCC 1K03195^T).

Keywords: Croceivirga litoralis sp. nov.; seawater; taxonomy.