Seroprevalence of fasciolosis in Hokkaido sika deer (Cervus nippon yesoensis) from Hokkaido Prefecture, Japan revealed by ELISA using recombinant cathepsin L1

Hironobu Sato; Hiroki Hiraya; Takutoshi Sugiyama; Shinya Fukumoto; Ryota Matsuyama; Yojiro Yanagawa; Ryo Nakao; Takao Irie; Kensuke Taira; Akiko Yamazaki; Katsuro Hagiwara; Ayako Yoshida; Yoichi Kamata; Madoka Ichikawa-Seki

doi:10.1016/j.parint.2020.102222

Seroprevalence of fasciolosis in Hokkaido sika deer (Cervus nippon yesoensis) from Hokkaido Prefecture, Japan revealed by ELISA using recombinant cathepsin L1

Parasitol Int. 2021 Feb:80:102222. doi: 10.1016/j.parint.2020.102222. Epub 2020 Oct 31.

Authors

Affiliations

¹ Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan.
² Laboratory of Veterinary Public health, Faculty of Agriculture, Iwate University, Morioka, Japan.
³ National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
⁴ Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
⁵ Laboratory of Theriogenology, Department of Clinical Sciences, Division of Veterinary Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
⁶ Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
⁷ Department of Infection Diseases, Hokkaido Institute of Public Health, Sapporo, Japan; Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
⁸ Laboratory of Parasitology, School of Veterinary Medicine, Azabu University, Sagamihara, Japan.
⁹ School of Veterinary Medicine, Rakuno-Gakuen University, Ebetsu, Japan.
¹⁰ Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Centre for Animal Diseases Control (CADIC), University of Miyazaki, Miyazaki, Japan.
¹¹ Laboratory of Veterinary Public health, Faculty of Agriculture, Iwate University, Morioka, Japan; Department of Food and Nutrition, Faculty of Human Life Science, Senri Kinran University, Suita, Japan.
¹² Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan. Electronic address: madoka@iwate-u.ac.jp.

PMID: 33137508
DOI: 10.1016/j.parint.2020.102222

Abstract

Fasciolosis, a zoonotic disease caused by liver flukes of the genus Fasciola, has been reported in Hokkaido (Yezo) sika deer (Cervus nippon yesoensis) in Hokkaido Prefecture, Japan; however, the actual seroprevalence in the animal has not been adequately evaluated. The objective of the present study was to analyze the seroprevalence of the disease among Hokkaido sika deer. Recombinant cathepsin L1 (rCatL1) was used as an antigen for an indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Fasciola flukes. The sensitivity and specificity of the ELISA were 84.6% and 100%, respectively. The average seroprevalence in 1109 Hokkaido sika deer from 20 locations in Hokkaido Prefecture was 43.9%. Mature deer showed higher seroprevalence than younger individuals; however, even younger animals may act as a reservoir for the disease. Monitoring infection levels in the Hokkaido sika deer population is important not only for the livestock industry, but also for preventing human fasciolosis.

Keywords: Cathepsin L1; ELISA; Fasciolosis; Hokkaido sika deer; Seroprevalence.

MeSH terms

Animals
Antigens, Helminth / analysis*
Cathepsins / analysis*
Deer*
Enzyme-Linked Immunosorbent Assay / methods
Enzyme-Linked Immunosorbent Assay / veterinary*
Fascioliasis / epidemiology
Fascioliasis / parasitology
Fascioliasis / veterinary*
Japan / epidemiology
Prevalence
Recombinant Proteins / analysis
Sensitivity and Specificity
Seroepidemiologic Studies

Substances

Antigens, Helminth
Recombinant Proteins
Cathepsins
cathepsin L1, Fasciola hepatica