Identification and characterization of the first fish parvalbumin-like protein data from a pathogenic fungal species, Trichophyton violaceum

Reza Zolfaghari Emameh; Leila Masoori; Hassan Nosrati; Reza Falak; Seppo Parkkila

doi:10.1016/j.dib.2020.106420

Identification and characterization of the first fish parvalbumin-like protein data from a pathogenic fungal species, Trichophyton violaceum

Data Brief. 2020 Oct 19:33:106420. doi: 10.1016/j.dib.2020.106420. eCollection 2020 Dec.

Authors

Reza Zolfaghari Emameh¹, Leila Masoori², Hassan Nosrati³, Reza Falak⁴, Seppo Parkkila^{5

6}

Affiliations

¹ Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965/161, Tehran, Iran.
² Department of Laboratory Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.
³ Department of Materials Engineering, Tarbiat Modares University, Tehran, Iran.
⁴ Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran.
⁵ Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
⁶ Fimlab Ltd. and Tampere University Hospital, FI-33520 Tampere, Finland.

Abstract

Parvalbumins are the most important fish allergens, which are heat-stable, classified in the family of calcium-binding EF-hand proteins, and contain one magnesium binding site. The functional connection between calcium and parvalbumin gives fish the high-speed swimming ability because of high concentration of Ca²⁺-binding parvalbumin in fish white muscles. Although parvalbumins are widely studied and conceivably play crucial roles in the physiology and swimming pattern of fishes, still no report is available about their presence in microbes, such as pathogenic fungal species. We detected a DNA sequence in the genome of Trichophyton violaceum and used in silico and polymerase chain reaction (PCR) technique with a designed pair of primers to identify it as parvalbumin-coding gene.

Keywords: DNA sequence; Fungi; In silico; Molecular diagnosis; Parvalbumin; Polymerase chain reaction (PCR); Trichophyton violaceum.