An Aurora Kinase B-Based Mouse System to Efficiently Identify and Analyze Proliferating Cardiomyocytes

Wenbin Fu; Qiao Liao; Liangpeng Li; Yu Shi; Andi Zeng; Chunyu Zeng; Wei Eric Wang

doi:10.3389/fcell.2020.570252

An Aurora Kinase B-Based Mouse System to Efficiently Identify and Analyze Proliferating Cardiomyocytes

Front Cell Dev Biol. 2020 Oct 7:8:570252. doi: 10.3389/fcell.2020.570252. eCollection 2020.

Authors

Wenbin Fu^{1

2

3

4}, Qiao Liao^{1

2

3}, Liangpeng Li^{1

2

3}, Yu Shi^{1

2

3}, Andi Zeng^{1

2

3}, Chunyu Zeng^{1

2

3

5}, Wei Eric Wang^{1

2

3}

Affiliations

¹ Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, China.
² Department of Cardiology, Daping Hospital, Army Medical University, Chongqing, China.
³ Chongqing Institute of Cardiology, Chongqing, China.
⁴ Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, China.
⁵ Cardiovascular Research Center, Chongqing College, University of Chinese Academy of Sciences, Chongqing, China.

Abstract

To identify and analyze the live proliferating cardiomyocytes is crucial for deciphering the mechanisms controlling endogenous cardiac regeneration. Traditional methods confuse cell division with multinucleation in postnatal cardiomyocytes. Recent efforts have achieved significant progress on discerning cytokinesis from only nuclear division. However, those methods were either designed to label post-cytokinesis progeny or challenging to sort the live proliferating cardiomyocytes. In this study, we highlighted an Aurora kinase B reporter-based mouse system with a tdTomato fluorescence labeling. It could efficiently identify proliferating cardiomyocytes in neonates. The analysis of sorting tdTomato⁺ cardiomyocytes with different ploidy indicated that mononucleated cardiomyocytes might not possess significantly higher proliferating potential than other cardiomyocytes when most cardiomyocytes have become post-mitotic. Moreover, tdTomato⁺ cardiomyocytes were significantly increased and enriched at injury border zone after apex resection in neonates, while there were no increased tdTomato⁺ cardiomyocytes after myocardial infarction in adults.

Keywords: Aurora kinase B; development; mice; myocardium; regeneration.