Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine

Bert Dhondt; Nicolaas Lumen; Olivier De Wever; An Hendrix

doi:10.1016/j.xpro.2020.100073

Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine

STAR Protoc. 2020 Jul 27;1(2):100073. doi: 10.1016/j.xpro.2020.100073. eCollection 2020 Sep 18.

Authors

Bert Dhondt^{1

2

3}, Nicolaas Lumen^{2

3}, Olivier De Wever^{1

2}, An Hendrix^{1

2}

Affiliations

¹ Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.
² Cancer Research Institute Ghent, Ghent, Belgium.
³ Department of Urology, Ghent University Hospital, Ghent, Belgium.

Abstract

The multidimensional cargo of extracellular vesicles (EV) released in urine is a reflection of the pathophysiological processes occurring within their cells and tissues of origin in the urogenital system. Here, we describe a step-by-step protocol for density-based separation of urinary EV with high specificity and repeatability. The implementation of integrative omics allows the study of the molecular complexity of highly purified urinary EV, supporting the identification of EV-specific functions and biomarkers. For complete details on the use and execution of this protocol, please refer to Dhondt et al. (2020).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / urine*
Chemical Fractionation / methods*
Extracellular Vesicles / chemistry*
Humans
Proteomics
Reproducibility of Results

Substances

Biomarkers