A hepatocyte-targeting fluorescent probe for imaging isoniazid-induced hydrazine in HepG2 cells and zebrafish

Chem Commun (Camb). 2020 Nov 25;56(91):14183-14186. doi: 10.1039/d0cc05330a. Epub 2020 Oct 27.

Abstract

A hepatocyte-targeting fluorescent N2H4 probe, GHP, was first designed and synthesized employing N-acetylgalactosamine (GalNAc) as the hepatocyte-targeting group and 3-nitrophthalimide as the recognition moiety. The probe can be used to selectively image N2H4 produced by the hydrolysis of isoniazid in HepG2 cells and the liver of zebrafish in situ.

MeSH terms

  • Animals
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Hep G2 Cells
  • Hepatocytes / chemistry*
  • Hepatocytes / metabolism
  • Humans
  • Hydrazines / analysis*
  • Hydrazines / metabolism
  • Hydrolysis
  • Isoniazid / chemistry*
  • Isoniazid / metabolism
  • Molecular Structure
  • Optical Imaging*
  • Zebrafish

Substances

  • Fluorescent Dyes
  • Hydrazines
  • hydrazine
  • Isoniazid